Abstract

An arylsulfatase is defined as a lysin of sea urchin sperm from the following evidences. (1) The activity is detected in the sperm of all the sea urchins investigated, and the activity is partially liberated from the cells after the acrosome reaction ( Moriya and Hoshi, 1979). (2) Fertilization is completely inhibited in the presence of 40 m Mp-nitrophenyl sulfate, which is an artificial substrate of arylsulfatase, but is not inhibited by p-nitrophenyl phosphate at the same concentration. (3) The inhibitory effect of p-nitrophenyl sulfate on fertilization is remarkably diminished by pretreatment of eggs with arylsulfatase before insemination. (4) Sperm arylsulfatase as well as limpet arylsulfatase appear to digest the vitelline coat and jelly coat.

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