Arylsulfatase activity of soil microbial biomass along a Mediterranean-arid transect

Abstract

The relationships between arylsulfatase and microbial activity were investigated in regional and microenvironmental scales, at three study sites in Israel, that represent different climatic regions—Mediterranean (sub-humid), mildly arid and arid. Total arylsulfatase activity was divided into extracellular and intracellular (microbial biomass enzyme) activities according to the chloroform-fumigation method. The results show that with increasing aridity, C org (soil organic carbon), C mic (soil microbial biomass carbon), N mic (soil microbial biomass nitrogen) and respiration rate decreased, while C mic/C org and metabolic quotient (qCO 2) increased. Total, extracellular and microbial biomass arylsulfatase activities decreased with aridity. Expressed as percentage of total activity, the arylsulfatase activity of microbial biomass in the soil, at 0–2 cm and 5–10 cm depths, accounted for more than 50% of the total, in most measurements. This activity was significantly higher in the arid sites than that found in the Mediterranean one for the 0–2 cm soil. The results indicate the importance of the microflora as an enzyme source in soils, especially in arid climate conditions. Enzyme activity in the different study sites was found to be influenced by microenvironmental conditions. The Mediterranean site showed a much higher enzyme activity under shrubs than that under rock fragments and in bare soil. In the arid site rock fragments created a favorable microenvironment for microbial activity on soil surface, which resulted in a much higher microbial biomass and arylsulfatase activity than that in bare soil. The total, extracellular and intracellular arylsulfatase activities, were significantly correlated with C org, C mic, N mic and respiration rate ( p<0.05) at all study sites. The correlation coefficients between microbial biomass and arylsulfatase activity were usually higher than those between organic carbon and enzyme activity, especially in the arid sites. Close relationships between microbial biomass and arylsulfatase activities in all the studied sites supported the hypothesis that C org content and enzyme activities should be related to each other via microbial biomass. Arylsulfatase activity was found to be a good indicator of microbial one. The regression equations between these factors can be incorporated into models of biogeochemical cycling for their easy method of analysis.