Artificial spawning of female Polish line 3 carp (Cyprinus carpio L.) after treatment with pituitary homogenate and/or Ovopel

Abstract

Stimulation of ovulation was carried out in female carp (Cyprinus carpio L.) of Polish line 3, with carp pituitary homogenate, Ovopel (the preparation contains d-Ala6, Pro9NEt-mGnRH and metoclopramide), and Ovopel and carp pituitary homogenate. The effect of the ovulation stimulators on the weight of eggs expressed in grams and as a percentage of female body weight was determined. The effect of the treatments on the quality of eggs expressed as the percentage of living embryos after 24- and 36-h incubation was also determined. The best results were obtained after stimulation with Ovopel (the priming dose) and pituitary homogenate (the resolving dose). In this case all the females spawned, and the highest yield and quality of eggs were recorded. The poorest results were noted if the ovulation was stimulated with repeated injection of carp pituitary homogenate. The females treated with carp pituitary homogenate gave eggs of poorer quality than those stimulated with two doses of Ovopel or with Ovopel and pituitary homogenate. The percentage of living embryos after 24- and 36-h incubation of eggs was significantly higher for the group stimulated with Ovopel and pituitary homogenate than for the other treatments.