Abstract
An experiment was performed to observe the potentiality of synthetic hormone analogue in artificial propagation i.e., the embryonic and larval development of Heteropneustes fossilis. Broodfish were injected with S-GnRHa according to the following concentration: 1, 2.5 and 5 ml kg-1 of body weight (BW) to females and 0.5, 1.125 and 2.5 ml kg-1 BW to males, in treatment groups T1, T2 and T3, respectively each with three replicates. The fishes were ovulated at about 10-11 hours after the hormone injection. Result showed the highest fertilization (83.11±1.36) and hatching rate (89.56±1.04) in T2, whereas ovulation rate was 100% in all treatment groups. First cleavage was observed in 30 minutes of post-fertilization. Embryonic developmental period sequentially for 2-cell, 4-cell, 8-cell, 16-cell, 32-cell, 64-cell, Morula, Blastula, Gastrula, somatic formation, yolk-plug, twisting movement and pre-hatching were 00:30, 00:45, 01:10, 01:30, 02:00, 02:30, 03:00, 04:00, 06:40, 09:00-18:00, 19:00, 20:00-21:00 and 22:00 hours, respectively. Hatching occurred after 23:00 hours of fertilization. Finally, the current result suggested that S-GnRHa might be an effective synthetic hormone in artificial propagation of H. fossilis.
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