Abstract
This work mainly focused on the production of an efficient, economical, and eco-friendly lipase (AKL29) from Actinomadura keratinilytica strain Cpt29 isolated from poultry compost in north east of Algeria, for use in detergent industries. AKL29 shows a significant lipase activity (45 U/mL) towards hydrolyzed triacylglycerols, indicating that it is a true lipase. For maximum lipase production the modeling and optimization of potential culture parameters such as incubation temperature, cultivation time, and Tween 80 (v/v) were built using RSM and ANN approaches. The results show that both the two models provided good quality predictions, yet the ANN showed a clear superiority over RSM for both data fitting and estimation capabilities. A 4.1-fold increase in lipase production was recorded under the following optimal condition: incubation temperature (37.9 °C), cultivation time (111 h), and Tween 80 (3.27%, v/v). Furthermore, the partially purified lipase showed good stability, high compatibility, and significant wash performance with various commercial laundry detergents, making this novel lipase a promising potential candidate for detergent industries.
Highlights
Lipases are glycerol ester hydrolases that catalyzes the hydrolysis of triacylglycerols to release diacylglyceride, monoacylglycerol, long-chain fatty acids and glycerol at the interface of oil and water.[1]
This work mainly focused on the production of an efficient, economical, and eco-friendly lipase (AKL29) from Actinomadura keratinilytica strain Cpt[29] isolated from poultry compost in north east of Algeria, for use in detergent industries
This study mainly focuses on the optimization of the parameters of culture medium to increase the production of AKL29
Summary
Lipases are glycerol ester hydrolases that catalyzes the hydrolysis of triacylglycerols to release diacylglyceride, monoacylglycerol, long-chain fatty acids and glycerol at the interface of oil and water.[1] It has been reported that the first lipases were obtained from Penicillium oxalicum and Aspergillus flavus.[2] Since, the lipases were considered as a great biotechnological and industrial catalyst after carbohydrases and proteases.[3] Lipases are prevalent in nature and are produced by plants, animals, and microorganisms including fungi, bacteria, and actinomycetes.[4] Recently, it has been reported that several actinomycete isolates are able to hydrolyze fats and oils.[5] Microbial lipases are mainly extracellular and their production is significantly influenced by the culture medium parameters. The major factor influencing the lipase activity was the carbon source. The production of these lipases is generally conducted in the presence of oil, triacylglycerols, fatty acids, esters, glycerol, or Tweens.[6,7] Microbial lipases play a major role in various fields such as the synthesis of organic chemicals and industrial applications.
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