Abstract
Studies on dilute sulphuric acid pretreatment of water hyacinth for xylose yield was carried out with four independent parameters namely temperature, concentration, treatment time and residence time. Response surface methodology (RSM) was implemented to develop an experimental design matrix. Artificial neural network modelling was studied to develop and optimise the process based on the results obtained from the RSM design. The xylose yield under optimised condition obtained experimentally was 164.76 mg/g of dry water hyacinth biomass when hydrolysed with 4.89/ sulphuric acid, at 130°C operating temperature, residence time of 59.67 minutes and treatment time of ten minutes. Enzymatic saccharification was followed using a cocktail of cellulase and xylanase enzyme, which resulted in a total reducing sugar yield of 396.34 mg/g which followed fermentation. A substantial yield of 6.84 g/L of ethanol was obtained using P. stipitis the hydrolysate derived from hydrolysis.
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