Abstract
Enhanced understanding of cellular microRNA (miRNA) biogenesis machinery has allowed researchers to engineer synthetic or artificial miRNAs (amiRNAs) that can be designed to direct efficient silencing of any transcript. The amiRNA technology has not only widened the existing gene silencing tool kit but also offers several distinct improvements over existing RNAi approaches, primarily based on siRNA generating hairpin RNA precursors. amiRNAs have already been applied to a wide range of agricultural and medical applications. This chapter discusses various aspects of miRNA processing, design principles of amiRNA expression vectors and their application.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
