Abstract
Evaluation of four different methods of artificial inoculation viz., syringe and spindle inoculation, detached leaf assay and sett dip methods indicated that the syringe method was the most suitable followed by spindle inoculation for evaluation of sugarcane varieties against pokkah boeng disease under controlled conditions. However, the preliminary assessment of the resistance of 88 sugarcane germplasm lines against two most prevalent fungal species associated with pokkah boeng disease, F. verticillioides CNO-1 and F. proliferatum YN-41, by spindle inoculation method indicated that the CNO-1 was more virulent. Furthermore, our study identified GT11, GT37, NCO310, POJ2878, and F134 as highly susceptible, whereas CP84-1198, GT05-3846, ROC27, and YT94-128 as highly resistant clones. Spindle inoculation method was found as the most reliable method of inoculation of young sugarcane germplasm under indoor condition for screening resistance against pokkah boeng disease.
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