Abstract
Abundance of humic substances (HS) are the secret of fertile soils, but not all soils have enough humic matter, also due to anthropogenic over-cultivation. Artificial humic acid (A-HA) and artificial fulvic acid (A-FA) were synthesized from agricultural litter, with high similarity to natural extracts. These samples were added to black soils, and soil activity and nutrients availability were analyzed. The results demonstrate that the content of dissolved organic matter (DOC) and total organic carbon (TOC) largely increased. The increment of C in the soil was up to 21.4 g/kg, much higher than the carbon added via the A-HA. As a “secondary” benefit, nutrient availability is increased, promoting the growth of plants. Using high-throughput sequencing we revealed that A-HA strongly supports the growth of a photosynthetic Rubrivivax gelatinosus, which induced the carbon sequestration. Thus, application of A-HS shows potential for biologically amplified carbon sequestration within black soils.
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