Abstract
The present study evaluated the effect of artificial gravity loading on transporter-mediated uptake and release of L-glutamate using the inhibitors of glutamate transporters as tools. The competitive nontransportable, DL-threo-beta-benzyloxyaspartate (DL-TBOA), and transportable, DL-threo-beta-hydroxyaspartate (DL-THA), inhibitors were demonstrated to better inhibit the L-[14C]glutamate uptake under centrifuge-induced hypergravity compared with the normal gravity control. The effect of DL-TBOA on depolarization-induced carrier-mediated L-[14C]glutamate release also increased after hypergravity loading in Na+- and low [Na+] NMDG- supplemented media. 10 µM DL-TBOA-induced decrease in L-[14C]glutamate release in Na+ — supplemented medium was 15.2±2.2 % in the control experiments and 26.2±3.9 % after centrifuge-induced loading (P≤0,05) and in low [Na+] medium was 37.0±2.5 % and 45.0±3.4 %, respectively.
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