Abstract
The last decade has seen a dramatic increase in the application of FT-IR spectroscopy to biological problems. As well as transmission methods, many specialized techniques are increasingly being applied to biological structural studies, the most popular being ATR spectroscopy. While ATR-IR spectroscopy has been applied to conformational studies of lipids and membrane-associated proteins and peptides for some time, it has only recently been applied to soluble proteins. Two assumptions are made in the analysis of soluble proteins as films. First, it is assumed that drying of films does not result in irreversible structural alterations. Second, it is assumed that rehydration of the film results in the same degree of protein/solvent interaction as in solution, or that reduced solvent interaction in films does not produce structural alterations. In this note we compare spectra of representative proteins as films deposited on ATR elements and in solution. Our results show that significant differences are apparent between solution and film spectra.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
