Abstract
Bone metabolism mainly depends on the osteoblasts and osteoclasts which are involved in the bone formation and resorption, respectively. Osteoclasts are multinucleated giant cells differentiated from the precursors of monocyte/macrophage cell lineage. Ribosome-inactivating proteins (RIPs) are a group of proteins with characteristics of blocking the function of ribosome. Recombinant articulatin is part of Type-2 ribosome inactivating protein. Type-2 ribosome inactivating protein consists of an A chain and a B chain. Among them, the B chain is a kind of lectins, specific for sugars with galactose moiety. In this study, the role of B chain of recombinant articulatin in the osteoclast formation was elucidated. We used the tartrate-resistant acid phosphatase (TRAP) staining and the artificial bone cell culture system to evaluated the osteoclast fromation and bone resorption, respectively. The results demonstrated that RAW264.7, a precursor of murine macrophages, can be differentiated into osteoclast-like cell in the presence of the recombinant articulatin B-chain, using the TRAP and artifical bone cell culture as the identified marker and activity for osteoclast, respectively. Therefore, in addition to being as a ribosome inhibitor, a novel role for Type-2 ribosome inactivating protein play an important role in the differentiation process from monocyte/macrophage precursors to osteoclasts is revealed in this study.
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