Abstract
Zymomonas mobilis is a promising microbial chassis for the production of fuels and chemicals from energy crops. However, the lack of efficient genome engineering tools limits synthetic biology applications. This article from Lal et al. (e00808-21) describes the role of several defense systems in this bacterium that control foreign DNA uptake. Strains designed to lack one or more restriction systems dramatically improve DNA uptake and will allow for more efficient genetic engineering in this bacterium.
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