Abstract
Solvent-tolerant bacteria constitute promising platforms for biobased production of aromatic compounds and biopolymer building blocks. In Pseudomonas putida, solvent tolerance is associated with megaplasmids carrying solvent pumps and aromatic degradation pathways. Kusumawardhani et al. (e00041-21) succeeded in restoring solvent tolerance in P. putida S12 lacking its megaplasmid, by using adaptive laboratory evolution (ALE). Availability of a promiscuous resistance-nodulation-division (RND) extrusion pump, combined with metabolic flexibility, proved essential. This work underscores the importance of ALE as a tool to identify targets for strain optimization in general and to enhance solvent tolerance in P. putida and in other non-solvent-tolerant bacteria.
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