Abstract

Artemisia anomala S. Moore is a perennial herbaceous plant classified as Asteraceae of the genus Artemisia. Many species of Artemisia have been used as medicinal materials. Artemisia anomala S. Moore has been widely used in China to treat inflammatory diseases. However, the mechanism of its action on the keratinocyte inflammatory response is poorly understood. Here, we investigated the anti-inflammatory reaction of Artemisia anomala S. Moore ethanol extract (EAA) using human keratinocyte (HaCaT) cells, which involved investigating the nuclear factor kappa B (NF-κB), signal transducer, and activator of transcription-1 (STAT-1), as well as mitogen-activated protein kinase (MAPK) signaling pathways and atopic dermatitis-like skin lesions in mice. We elucidated the anti-inflammatory effects of EAA on tumor necrosis factor-α/interferon-γ (TNF-α/IFN-γ)-treated human keratinocyte cells and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like mice. The levels of chemokines and cytokines (IL-8, IL-6, TARC, and RANTES) were determined by an enzyme-linked immunosorbent assay. The NF-κB, STAT-1, and MAPK signaling pathways in HaCaT cells were analyzed by western blotting. Thickening of the mice dorsal and ear skin was measured and inflammatory cell infiltration was observed by hematoxylin and eosin staining. Results showed that EAA suppressed IL-8, IL-6, TARC, and RANTES production. EAA inhibited nuclear translocation of NFκB and STAT-1, as well as reduced the levels of phosphorylated ERK MAPKs. EAA improved AD-like skin lesions in DNCB-treated mice. These findings suggest that EAA possesses stronger anti-inflammatory properties and can be useful as a functional food or candidate agent for AD.

Highlights

  • We investigated the effects of EAA in the DNCB-induced atopic dermatitis (AD) mouse model and TNF-α/IFN-γ-stimulated HaCaT cells

  • Activated keratinocytes can induce the upregulation of pro-inflammatory chemokines and chemokines, including interleukin-6 (IL-6), interleukin-8 (IL-8), regulated upon activation (RANTES; CCL5), and TARC; CCL17) [17]

  • Enzyme-Linked Immunosorbent Assay (ELISA) kits for IL-8 (#431501), RANTES (#440804), thymus and activation regulatory chemokines (TARC, #441104), IL-6 (#430501), recombinant human tumor necrosis factor alpha (TNF-α), and the interferon gamma (IFN-γ) were purchased from BioLegend (San Diego, CA, USA)

Read more

Summary

Introduction

Moore is called “Yu-Gi-no” in Korean medicine and ”Nan-Liu-Ji-. Nu” in Chinese medicine, and is a perennial herbaceous plant classified as the Artemisia genus in the Compositae family [1]. Moore has long been used in the treatment of diseases through modern medicine and traditional medicine, such as for dissipated liver function caused by hepatitis, fever, and inflammation in China, Japan and Korea [2]. Moore, such as quercetin, apigenin, and tricin, were reported for anti-inflammation, anti-oxidant and anti-cancer effects. Moore in atopic dermatitis (AD) in terms of its therapeutic effect are not clear [3,4]

Methods
Results
Discussion
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call