Abstract
Different developmental stages of Artemia spp. (metanauplii, juveniles and adults) were bath-challenged with two isolates of the Lymphocystis disease virus (LCDV), namely, LCDV SA25 (belonging to the species Lymphocystis disease virus 3) and ATCC VR-342 (an unclassified member of the genus Lymphocystivirus). Viral quantification and gene expression were analyzed by qPCR at different times post-inoculation (pi). In addition, infectious titres were determined at 8 dpi by integrated cell culture (ICC)-RT-PCR, an assay that detects viral mRNA in inoculated cell cultures. In LCDV-challenged Artemia, the viral load increased by 2–3 orders of magnitude (depending on developmental stage and viral isolate) during the first 8–12 dpi, with viral titres up to 2.3 × 102 Most Probable Number of Infectious Units (MPNIU)/mg. Viral transcripts were detected in the infected Artemia, relative expression values showed a similar temporal evolution in the different experimental groups. Moreover, gilthead seabream (Sparus aurata) fingerlings were challenged by feeding on LCDV-infected metanauplii. Although no Lymphocystis symptoms were observed in the fish, the number of viral DNA copies was significantly higher at the end of the experimental trial and major capsid protein (mcp) gene expression was consistently detected. The results obtained support that LCDV infects Artemia spp., establishing an asymptomatic productive infection at least under the experimental conditions tested, and that the infected metanauplii are a vector for LCDV transmission to gilthead seabream.
Highlights
The family Iridoviridae comprises two subfamilies and six genera [1]
The aim of the present study was to investigate the susceptibility of different developmental stages of Artemia spp. to Lymphocystis disease virus (LCDV) and to elucidate its role as a vector for LCDV transmission to gilthead seabream
To establish if LCDV replicates in Artemia spp. cells, experimental infections were carried out using LCDV SA25 and three developmental stages of Artemia
Summary
The family Iridoviridae comprises two subfamilies and six genera [1]. Three of them, Lymphocystivirus, Megalocytivirus, and Ranavirus (subfamily Alphairidovirinae), infect ectothermic vertebrates (amphibians, reptiles and bony fish), whereas the hosts for the other three genera, Iridovirus, Chloriridovirus, and Decapodiridovirus (subfamily Betairidovirinae), are invertebrates (primarily insects and crustaceans) [1,2,3].Members of the genus Lymphocystivirus, collectively named as Lymphocystis disease virus (LCDV), are the causative agents of the Lymphocystis disease (LCD) affecting a wide variety of freshwater, brackish, and marine fish species [4]. Lymphocystivirus, Megalocytivirus, and Ranavirus (subfamily Alphairidovirinae), infect ectothermic vertebrates (amphibians, reptiles and bony fish), whereas the hosts for the other three genera, Iridovirus, Chloriridovirus, and Decapodiridovirus (subfamily Betairidovirinae), are invertebrates (primarily insects and crustaceans) [1,2,3]. The characteristic lesions of LCD are small pearl-like nodules on fish skin and fins, grouped in raspberry-like clusters of tumorous appearance [5,6]. This disease is rarely fatal, affected fish cannot be commercialized, provoking important economic losses [7]. LCD is the main viral infection reported in gilthead seabream (Sparus aurata) aquaculture [8]
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