Abstract
Much effort has been put into the process to obtain undistorted biological specimens for use in electron microscopy. It has been well accepted that the main advantage of the freeze-etching method is to obtain cells stabilized in the living state. This method has proved to be a useful tool to study membrane structures. This communication deals with a number of artefacts which occur during the freeze-etching preparation.The effect of glycerol treatment as an anti-freeze agent on the cellular membranes has not yet been completely solved. The interpretation of results must take into account the physical treatment during the steps of preparation. In order to avoid decreased viability others have added glycerol incrementally; however, in some cases alterationsin cellular structure have been reported.
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