Arsenic and Prostate Cancer: Acquiring Androgen Independence

Abstract

Vol. 113, No. 9 EnvironewsOpen AccessArsenic and Prostate Cancer: Acquiring Androgen Independenceis accompanied byAcquisition of Androgen Independence by Human Prostate Epithelial Cells during Arsenic-Induced Malignant Transformation John Tibbetts John Tibbetts Search for more papers by this author Published:1 September 2005https://doi.org/10.1289/ehp.113-a614bCited by:3AboutSectionsPDF ToolsDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InReddit Scientists already suspect that prostate cancer, the second-leading cause of cancer death in U.S. men, is linked with chronic arsenic exposure. Now a team of researchers reports that human prostate cells that underwent chronic, low-level arsenic exposure not only exhibited aggressive carcinoma-like growth, but also showed an increased incidence of androgen independence, a state often linked to advanced or fatal prostate cancers, and one that makes these cancers more difficult to treat [EHP 113:1134–1139].Androgen, a sex hormone that stimulates and maintains masculine characteristics, is a necessary component in normal prostate function that can also encourage the survival and growth of prostate cancer. That is why some current treatments, such as pharmaceutical androgen blockers or removal of the testes, focus on making androgen less available to prostate cancer cells. However, some patients experience androgen independence, in which prostate cancer cells no longer need the male hormone to differentiate and grow out of control.The mechanisms behind androgen independence are not completely understood, but scientists do know that this phenomenon sometimes occurs when androgen receptors go functionally awry. In some cases, the androgen receptors are “overexpressed,” greatly increasing in number. In other cases, the receptors mutate, which can result in hyperresponsiveness to androgens. Still other receptor mutations allow nonandrogens (such as the estrogens) and even androgen inhibitors to stimulate cancer growth. Sometimes androgen receptors are bypassed completely, and cell growth is activated by other naturally occurring compounds including insulin-like growth factor-1, epidermal growth factor, and others.In the current study, researchers examined the growth of normal and arsenic-transformed human prostate epithelial cells. The arsenic-transformed cells had been exposed continuously to sodium arsenite and, after 29 weeks of exposure, produced malignant tumors when inoculated into nude mice. Both cell lines were observed in two different media. One medium included the complete range of steroids, including ample amounts of androgen and growth factors. The other lacked normal amounts of sex hormones and growth factors.The experiment showed that, consistent with malignant cell growth, prostate cells chronically exposed to arsenic grew more rapidly than control cells in both media. In the steroid-rich medium, the arsenic-transformed cells proliferated approximately twice as fast as the unexposed control cells. In the steroid-depleted medium, the arsenic-transformed cells proliferated about 2.5 times faster than control cells.Arsenic exposure therefore appeared to be associated with the acquisition of androgen independence in prostate cells. However, the observed arsenic-induced androgen independence did not occur by any previously studied mechanism; it was not linked to overexpression of androgen receptors or receptor mutations that facilitate cell growth via nonandrogens.A clue may lie in earlier experiments, in which the same researchers observed a marked increase in production of K-ras (an oncogene associated with prostate cancers) in arsenic-transformed cells. K-ras is a key part of a growth-stimulating pathway in the prostate that is stimulated by androgens. K-ras was clearly correlated with arsenic-induced carcinoma-like growth and androgen independence. The authors speculate here that arsenic may bypass the androgen receptor and directly cause aberrant K-ras activation.FiguresReferencesRelatedDetailsCited by McDonald A, Gernand J, Geyer N, Wu H, Yang Y and Wang M (2022) Ambient air exposures to arsenic and cadmium and overall and prostate cancer–specific survival among prostate cancer cases in Pennsylvania, 2004 to 2014, Cancer, 10.1002/cncr.34128, 128:9, (1832-1839), Online publication date: 1-May-2022. Polo A, Marchese S, De Petro G, Montella M, Ciliberto G, Budillon A and Costantini S (2018) Identifying a panel of genes/proteins/miRNAs modulated by arsenicals in bladder, prostate, kidney cancers, Scientific Reports, 10.1038/s41598-018-28739-6, 8:1, Online publication date: 1-Dec-2018. Bulka C, Jones R, Turyk M, Stayner L and Argos M (2016) Arsenic in drinking water and prostate cancer in Illinois counties: An ecologic study, Environmental Research, 10.1016/j.envres.2016.04.030, 148, (450-456), Online publication date: 1-Jul-2016. Related articlesAcquisition of Androgen Independence by Human Prostate Epithelial Cells during Arsenic-Induced Malignant Transformation5 May 2005Environmental Health Perspectives Vol. 113, No. 9 September 2005Metrics About Article Metrics Publication History Originally published1 September 2005Published in print1 September 2005 Financial disclosuresPDF download License information EHP is an open-access journal published with support from the National Institute of Environmental Health Sciences, National Institutes of Health. All content is public domain unless otherwise noted. Note to readers with disabilities EHP strives to ensure that all journal content is accessible to all readers. However, some figures and Supplemental Material published in EHP articles may not conform to 508 standards due to the complexity of the information being presented. If you need assistance accessing journal content, please contact [email protected]. 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