Arsenic accumulation pattern in water-soil-rice systems: A study of tolerance mechanisms and associated health risks

Abstract

Regardless of the daunting challenge of arsenic (As) contamination in Pakistan, literature on tolerance and responsible factors in paddy fields remain elusive. In this regard, we aimed to explore physiochemical factors responsible for As availability in water-soil-rice systems. The study highlighted rice defense mechanisms to mitigate As toxicity on growth and yield. In the present study, basmati rice samples were collected along with irrigation and soil samples from control (<10 μg/L), low (11–25 μg/L), medium (26–100 μg/L), and high (>100 μg/L) contaminated regions. Oxidative stress markers (MDA and H2O2) and antioxidant enzymatic assays (SOD, CAT, POD, APX) were measured by spectrophotometer. The Durov diagram was constructed by using Grapher software to identify prevalent water types in irrigation wells. Total As was measured in water, soil, and rice tissues by hydride generation-atomic absorption spectroscopy (HG-AAS). The Durov diagram showed that the majority of irrigation water was Ca-Mg-Cl type. Furthermore, the FTIR analysis identified different organic compounds, i.e., OH, CC, CI, and CBr, particularly in soil from high regions. The results indicated higher accumulation and translocation of As in the water-soil-rice system from a high region compared to control and other regions. Phenotypic traits, i.e., grain yield, biological yield, chlorophyll, and root parameters were significantly impacted under high As-contaminated region. A concentration-dependent increase was indicated in oxidative stress and antioxidant activities except for APX. Risk assessment indicated a higher hazard quotient (1.09) and carcinogenic risk (5.0 × 10−03) due to grain consumption in high As-contaminated regions. The present study emphasized the need for strict regulations and policies to mitigate As calamity at the local level and protect human health.