Arsenate toxicity on the apices of Pisum sativum L. seedling roots: Effects on mitotic activity, chromatin integrity and microtubules

Abstract

Arsenic (As) is one of the most toxic pollutants in the environment, where it severely affects both animal and plant growth. Despite the growing literature data on As effects on plant development, alterations induced by this element on meristem activity of the root have not been explored to any great extent. In the present study, short-term experiments with arsenate have been conducted on Pisum sativum L. seedlings to assess whether plant growth impairment is due to DNA/chromosome or mitotic microtubule damages. Root growth was studied by evaluating apical meristem activity and cell elongation. Mitotic aberrations, DNA fragmentation and microtubule organization of the apical cells were also analyzed. The results have shown that arsenate, at the lowest concentration (0.25 μM), slightly increases root growth and some related parameters, whilst the other concentrations have a dose-dependent negative effect on root growth, on the mitotic and labelling index (after bromo-deoxyuridine administration), and on the mitotic arrays of microtubule (through immunofluorescence). The main effects on mitosis occurred for 25 μM As. The percentage of metaphases increased, as did the irregular metaphases and c-mitoses. This was related to alterations in the mitotic spindles, which closely resemble those induced by colchicine. Chromosome breaks and ana/telophase bridges were virtually absent, whilst DNA fragmentation only increased from 25 μM arsenate onwards. These data point to a poor clastogenetic activity of As and implicate that microtubules are one of the main targets of As.