Array-Based Comparative Genomic Hybridization and Application

Abstract

Genomic DNA copy number alterations play a very important role in pathogenesis and development of many human diseases,such as tumors and genetic diseases.Only large copy number alterations can be detected by classic cytogenetic methods due to their limited resolution.Quantitative fluorescent PCR and fluorescence in situ hybridization require prior knowledge of the type and location of expected aberrations and can only be used for the analysis of a limited number of chromosomal loci at one time.Array-based comparative genomic hybridization can detect single copy loss,duplication,amplification and directly locate these genetic imbalances to genome because of its high resolution,sensitivity and high-throughput.It is an effective method for detecting genomic DNA copy number alterations in tumors,genetic diseases and normal population. Key words: Array-based comparative genomic hybridization; Genome; Copy number alterations