Abstract
This work compares the composition at different temperatures of gaseous phase of bergamot essential oil at equilibrium with the liquid phase. A new GC–MS methodology to determine quantitatively the volatile aroma compounds was developed. The adopted methodology involved the direct injection of headspace gas into injection port of GC–MS system and of known amounts of the corresponding authentic volatile compounds. The methodology was validated. This study showed that gaseous phase composition is different from that of the liquid phase at equilibrium with it.
Highlights
Phytotherapy employs fully characterized active ingredients extracted from plants for the treatment and prevention of many diseases.Essential oils and their components exhibit various biological activities and are used for human disease prevention and treatment
We studied the composition of the gaseous phase at equilibrium with the liquid phase of bergamot essential oil by developing a gas chromatography–mass spectrometry (GC–MS) method useful for the determination of the volatile aroma components
Our system could approximate the conditions under which aromatherapy is practiced. These results suggest that the determination of the gaseous phase composition in equilibrium with the liquid essential oil is critical for establishing the correlation between the volatile components and their activity
Summary
Phytotherapy employs fully characterized active ingredients extracted from plants for the treatment and prevention of many diseases.Essential oils and their components exhibit various biological activities and are used for human disease prevention and treatment. We studied the composition of the gaseous phase at equilibrium with the liquid phase of bergamot essential oil by developing a gas chromatography–mass spectrometry (GC–MS) method useful for the determination of the volatile aroma components.
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