Arginine Deiminase and Biotin Metabolism Signaling Pathways Play an Important Role in Human-Derived Serotype V, ST1 Streptococcus agalactiae Virulent Strain upon Infected Tilapia.

Yu Liu,Fangzhao Yu,Qunhong Gu,Ting Huang,Wanwen Liang,Rui Wang,Ming Chen,Zhiping Luo,Liping Li

doi:10.3390/ani10050849

Yu Liu, Fangzhao Yu + Show 7 more

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https://doi.org/10.3390/ani10050849

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Abstract

Simple SummaryPatients who were infected with Streptococcus agalactiae (ST1) were mainly associated with asymptomatic carriage. However, the invasive diseases in non-pregnant adults caused by S. agalactiae (serotype V, ST1) have increased recently. We have previously reported that human-derived S. agalactiae (serotype V, ST1) could infect tilapia with virulence and pathologic characteristics similar to highly virulent tilapia-derived S. agalactiae (ST7) strains. The potential risk of cross-species infection cannot be ignored. Therefore, our research provided a multi-omics analysis of the human-derived serotype V ST1 S. agalactiae strains, which were virulent and non-virulent to tilapia and provided a more comprehensive understanding of the virulence mechanism.Our previous study showed that human-derived Streptococcus agalactiae (serotype V) could infect tilapia, but the mechanism underlying the cross-species infection remains unrecognized. In this study, a multi-omics analysis was performed on human-derived S. agalactiae strain NNA048 (virulent to tilapia, serotype V, ST1) and human-derived S. agalactiae strain NNA038 (non-virulent to tilapia, serotype V, ST1). The results showed that 907 genes (504 up/403 down) and 89 proteins (51 up/38 down) were differentially expressed (p < 0.05) between NNA038 and NNA048. Among them, 56 genes (proteins) were altered with similar trends at both mRNA and protein levels. Functional annotation of them showed that the main differences were enriched in the arginine deiminase system signaling pathway and biotin metabolism signaling pathway: gdhA, glnA, ASL, ADI, OTC, arcC, FabF, FabG, FabZ, BioB and BirA genes may have been important factors leading to the pathogenicity differences between NNA038 and NNA048. We aimed to provide a comprehensive analysis of the human-derived serotype V ST1 S. agalactiae strains, which were virulent and non-virulent to tilapia, and provide a more comprehensive understanding of the virulence mechanism.

Highlights

Streptococcus agalactiae causes pneumonia and meningitis in humans, and causes streptococcosis in farmed tilapia [1,2,3]
Patients who were infected with S. agalactiae (ST1) were mainly associated with asymptomatic carriage, but the invasive diseases in non-pregnant adults caused by S. agalactiae have increased recently [7,8,9,10]
The results showed that NNA048 was virulent and NNA038 was avirulent to tilapia, which was consistent with our previous studies [11]

Summary

Introduction

Streptococcus agalactiae causes pneumonia and meningitis in humans, and causes streptococcosis in farmed tilapia [1,2,3]. Patients who were infected with S. agalactiae (ST1) were mainly associated with asymptomatic carriage, but the invasive diseases in non-pregnant adults caused by S. agalactiae (serotype V, ST1) have increased recently [7,8,9,10]. With the development of sequencing technologies, gene annotation of fish-derived S. agalactiae virulent strains has gradually been completed in whole or in part [22,23,24,25]. When compared with human-derived genomes, the key virulence factors of fish-derived S. agalactiae were different. Different serotypes of S. agalactiae from the same host have different key virulence factors [25,26,27,28]. The virulence mechanism of the cross-species infection of human-derived serotype V, ST1 S. agalactiae may be quite different from the previous studies, and needs further investigation

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