Arginine ammonification and L‐glutaminase assays as rapid indices of corn nitrogen availability

Abstract

AbstractIncreasing use of N fertilizer for crop production necessitates more rapid estimates on N provided by the soil in order to prevent under‐ or overfertilization and their adverse effect on plant nutrition and environmental quality. A study was conducted to investigate the responses of arginine ammonification (AA), L‐glutaminase activity (LG), soil N–mineralization indices, corn (Zea mays L.) crop–yield estimation, and corn N uptake to application of organic amendments. The relationships between corn N uptake and the microbial and enzymatic processes which are basically related to N mineralization in soil were also studied. The soil samples were collected from 0–15 cm depth of a calcareous soil that was annually treated with 0, 25, or 100 Mg ha–1 (dry‐weight basis) of sewage sludge and cow manure for 7 consecutive years. Soil total N (TN), potentially mineralizable N (N0), and initial potential rates of N mineralization (kN0) were significantly greater in sewage sludge–treated than in cow manure–treated soils. However, the amendment type did not influence soil organic C (SOC), AA, LG, and anaerobic index of N mineralization (Nana). The application rates proportionally increased N‐availability indices in soil. Corn N concentration and uptake were correlated with indices of mineralizable N. A multiple stepwise model using AA and Nana as parameters provided the best predictor of corn N concentration (R = 0.86, p < 0.001). Another model using only LG provided the best predictor of corn N uptake (R = 0.78, p < 0.001). This results showed that sewage‐sludge and cow‐manure application is readily reflected in certain soil biological properties and that the biological tests may be useful in predicting N mineralization and availability in soil.