Argentimetric assay of ranitidine in bulk drug and in dosage forms

Abstract

Two simple, rapid and cost-effective methods based on titrimetric and spectrophotometrictechniques are described for the assay of RNH in bulk drug and in dosage forms using silver nitrate, mer-cury(II)thiocyanate and iron(III)nitrate as reagents. In titrimetry, an aqueous solution of RNH is treatedwith measured excess of silver nitrate in HNO 3 medium, followed by determination of unreacted silvernitrate by Volhard method using iron(III) alum indicator. Spectrophotometric method involve the addi-tion a known excess of mercury(II)thiocyanate and iron(III)nitrate to RNH, followed by the measurementof the absorbance of iron(III)thiocyante complex at 470 nm. Titrimetric method is applicable over 4-30mg range and the reaction stoichiometry is found to be 1:1 (RNH: AgNO 3 ). In the spectrophotometricmethod, the absorbance is found to increase linearly with concentration of RNH which is corroboratedby the correlation coefficient of 0.9959. The system obey Beer’s law for 5-70 μg mL -1 . The calculatedapparent molar absorptivity and sandell sensitivity values are found to be 3.27 × 10 3 L mol -1 cm -1 , 0.107μg cm -2 respectively.