Abstract

To evaluate the chemical characteristics of grape and orange juices, and their erosive potential in the decrease of microhardness and the loss of enamel structure. Five grape and orange juices were evaluated for pH, titratable acidity, calcium, phosphate, and fluoride concentration. De-ionised water and Cola soft drink were used as a negative and positive control, respectively. Twelve specimens of bovine enamel were immersed in beverages for 10min at 37°C, 3 times/day for 7days. Erosive potential was quantified using microhardness and loss of enamel structure. Anova One Way, Student's t test, Multiple Regression and Spearman Correlation (p<0.05) were used to analyse the results. Powdered grape juice showed the lowest pH (3.18±0.03) and pure grape juice presented the highest titratable acidity (5.48±0.06mL NaOH/100mL). Fresh orange juice and soya-based grape juice revealed the lowest calcium (0.77±0.12mmol/L) and phosphate concentrations (0.35±0.06mmol/L), respectively. Among juices, powdered orange juice caused the greatest decrease in surface microhardness (SMH) (127.99±40.47ΔSMH) and grape juice from concentrate caused the greatest loss of enamel structure (13.30±3.56μm). All of the evaluated juices contributed to dental erosion. Grape juices presented greater erosive potential than orange juices. Pure, powdered and concentrated grape juices showed similar loss of enamel structure to the Cola soft drink. The erosive potential of beverages was statistically correlated to pH, titratable acidity, calcium, phosphate and fluoride concentrations.

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