Abstract
Demilunes have historically been considered to be integral components of the secretory tree of mixed salivary glands. Based on preservation of rat sublingual glands by rapid freezing and freeze-substitution fixation, Yamashina et al. have advanced the notion that serous demilunes are artifactual products of chemical fixation, that swollen mucous cells cause a relocation of serous cells to the endpiece periphery. This concept has been uncritically accepted by several authors of histology textbooks. This note presents a compendium of observations and unanswered questions that render this proposition untenable.
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