Are cysteine, glutathione and phytochelatins responses of Myriophyllum alterniflorum to copper and arsenic stress affected by trophic conditions?

Abstract

The aim of this article is to study the impact of both copper (Cu2+) and arsenic (As (V)) at 100µg/L, with each element being combined with trophic conditions at the level of glutathione, cysteine and phytochelatins in the aquatic macrophyte Myriophyllum alterniflorum, whose potential for bioindication and phytoremediation of metal/metalloid pollution has already been demonstrated. To achieve this goal, a synthetic medium, of a composition similar to the water found in the Vienne River in France's Limousin Region and modified for eutrophic or oligotrophic conditions, is prepared. The analysis of cysteine, glutathione and phytochelatins is performed at 0, 3, 7, 14 and 21 days. Our results indicate that the eutrophic medium without contaminant only induces a significant increase in the glutathione level when compared to the oligotrophic medium. However, the joint presence of As (V) and Cu is able to increase the synthesis of cysteine, glutathione and phytochelatins (PC2 and PC3) under both eutrophic and oligotrophic conditions, with a significant increase in the eutrophic medium compared to the oligotrophic one. Phytochelatins (PC2 and PC3) are induced after as little as 3 days of exposure to copper and arsenic under both trophic conditions. Copper induces the synthesis of more PC3 than PC2, unlike arsenic. Our results confirm the potential use of phytochelatins as a specific biochemical biomarker for metal/metalloid stress. In conclusion, the eutrophic condition combined with copper or arsenic does change the response of Myriophyllum alterniflorum by enhancing its antioxidative defense. Thus, M. alterniflorum phytochelatins represent a potential dedicated biomarker to monitor water quality in terms of metal/metalloid stress regardless of the trophic level.