Abstract
Previous work has shown strong interactions between pig gastric mucins and a highly deacetylated chitosan. Recently, mucins purified from different areas of the porcine stomach have been shown to differ in terms of their oligosaccharide substitution and net charge. How this regional variation may affect the properties of these mucins is of great interest in terms of the specificity of mucoadhesion with chitosan. We have investigated the interaction of a chitosan of degree of acetylation F A ≈ 0.11 with three different mucins purified from different areas of the porcine stomach (cardia region, corpus and antrum) at two different ionic strengths. Using sedimentation velocity in the analytical ultracentrifuge equipped with a schlieren optical system coupled on line to a CCD camera, the amount of chitosan interacting with mucin was determined. The degree of interaction varies between the three mucins with those from the cardiac region displaying the highest degree of interaction; in the case of the corpus and antrum, however, the interaction increases with increase in ionic strength, implying that the interaction between mucins and chitosans may have a hydrophobic as well as an electrostatic basis.
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