Are all motivation tests the same? The effect of two adaptations to a three-chamber consumer demand study in ferrets

Abstract

Ferrets, Mustela putorius furo, are increasingly used in infectious disease studies, particularly in influenza research. Which specific housing conditions and environmental enrichments are of particular importance for ferrets have not been part of a systematic evaluation. The motivation ferrets showed to reach different enrichments was assessed in multiple consumer demand study set-ups. To address the question whether these consumer demand set-ups give similar results, we assessed the effects of two ways of offering enrichments concurrently instead of consecutively. Six ovariectomized female ferrets were successively tested in a seven-chamber (7Ch), three-chamber (3Ch) and three-chamber ‘all-but-one’ (ABO) set-up. We compared the maximum price paid, visit number, visit duration and interaction time with the enrichments in the 3Ch versus the 7Ch and ABO set-ups, respectively. Compared to the 3Ch set-up, the ferrets in the ABO and 7Ch set-up showed a lower motivation to access, paid fewer and shorter visits to and interacted less with the enrichments. In the 7Ch, the ferrets especially showed a lower motivation for the less preferred enrichments and the empty chamber. These findings indicate that testing all the enrichments concurrently in the 7Ch set-up forced the ferrets to make more economic decisions, thereby providing more valuable information on how different enrichments are valued relative to one other. Adding preferred enrichment items to the home chamber, as was done in the ABO set-up, might have reduced the motivation to access or look for additional enrichment items. However, this set-up might not have a closed economy, making the ABO set-up unsuitable. Based on these findings, we advise testing all the enrichment categories concurrently instead of consecutively and keeping the number of items in the home cage to a minimum when performing a consumer demand study, as this appears the most optimal set-up to determine motivational priorities for resources in ferrets.