Abstract
Canine sperm evaluation is routinely assessed by optic microscopy, spectrophotometer, computer assisted semen analysis and sometimes flow cytometry. These methods can be time-consuming and are not always available for canine reproduction professionals. Furthermore, it is possible that a dog shows bad fertility despite a promising sperm quality. This is why new tools are studied in order to predict the quality and the fertility of a semen. Proteomic research is actually trying to identify some protein markers related to the fertility in the semen.
Highlights
Laboratory of Biotechnology and Reproductive Pathology, ONIRIS: The National Veterinary, Food Agriculture, and Food Hygiene School of Loire Atlantique, BP 40706, 44307 Nantes, France
The aim of this study is to identify proAKAP4 in dog testicle and analyze its expression in the ejaculate of this specie
A second 20 kDa protein has been detected in those fractions: it corresponds to the prodomain of the proAKAP4
Summary
Laboratory of Biotechnology and Reproductive Pathology, ONIRIS: The National Veterinary, Food Agriculture, and Food Hygiene School of Loire Atlantique, BP 40706, 44307 Nantes, France. Capacitation occurs in the female genital tract: it gives to the spermatozoa the ability to fertilize This event depends on an intracellular signalization mechanism including a calcium influx and phosphorylation of tyrosine residues on flagellar proteins. AKAP4 is found on the entire principal piece of the flagellum, in the longitudinal columns and in transverse ribs [14] It is described in the fibrous sheath in spermatozoa from mouse [15], bull [16], man [12], stallion [17] and boar [18]. Nevertherless, the absence of AKAP4 causes a flagellar disorganization: the fibrous sheath is not developed, the principal piece and the flagellum are shorter These spermatozoa are immobile: So they are infertile. The altered structure of the fibrous sheath is no longer available to organize the spermatic motility signals [19]
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