Arbuscular mycorrhizal symbiosis alleviates ozone injury in ozone-tolerant poplar clone but not in ozone-sensitive poplar clone

Abstract

Tropospheric ozone (O3) is a typical air pollutant with harmful effects on plants, whereas arbuscular mycorrhizal (AM) fungi are ubiquitous plant symbionts that enhance plant resistance to various abiotic stresses. However, whether AM symbiosis decreases plant O3 sensitivity and what the underlying mechanisms are remain unclear. In this study, O3-tolerant poplar clone 107 and O3-sensitive poplar clone 546 were used as test plants. An open-top chamber experiment was conducted to investigate the effects of AM inoculation on plant growth and physiological parameters under O3 enrichment. The results showed that O3 enrichment significantly decreased plant biomass and net photosynthetic rate and increased the leaf shedding rate and malondialdehyde concentration of clone 546. Generally, clone 107 was less responsive to O3 enrichment than clone 546 was. Differences in antioxidant enzyme activity, rather than in specific leaf weight or stomatal conductance, were responsible for the differences in O3 sensitivity between the two clones. AM inoculation significantly increased the biomass and decreased the leaf shedding rate and malondialdehyde concentration of clone 107 but had no significant effect on almost all the indexes of clone 546, suggesting a species-specific mycorrhizal effect on plant O3 sensitivity. Mechanistically, AM symbiosis did not significantly affect nutrient uptake, stomatal conductance, or specific leaf weight of poplar but did significantly increase antioxidant enzyme activity. Linear regression analysis of antioxidant enzyme activities and the effect of O3 on growth and physiological parameters showed that AM symbiosis mediated antioxidant enzyme activities to mitigate O3 injury to the two poplar clones. This study improved the understanding of the protective effects of AM fungi on plants against O3 pollution.