ARBUSCULAR MYCORRHIZAL FUNGI IN SEEDLING FORMATION OF BARBADOS CHERRY (Malpighia emarginata D.C.)

Eduardo Mendonça Pinheiro,Thayanna Vieira Costa,Camila Pinheiro Nobre,José Ribamar Gusmão Araujo,Orlando Carlos Huertas Tavares

doi:10.1590/1983-21252019v32n210rc

Eduardo Mendonça Pinheiro, Thayanna Vieira Costa + Show 3 more

Open Access

https://doi.org/10.1590/1983-21252019v32n210rc

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Abstract

ABSTRACT The use of beneficial microorganisms such as arbuscular mycorrhizal fungi (AMF) may favor both the growth phase and the stabilization of the seedlings after transplantation. The aim of this study was to evaluate the effect of inoculation of different AMF species on the development of Barbados cherry seedlings from herbaceous and semi-hardwood cuttings. Softwood and semi-hardwood cuttings, previously rooted, were planted in 500 ml tubes filled with Plantmax® substrate and inoculated with three species of mycorrhizal fungi (Gigaspora margarita - Gimarg, Claroideoglomus etunicatum - Claetun and Glomus clarum - Glclar) isolated and combined (Gimarg + Claetun, Gimarg + Glclar, Claetun + Glclar and Gimarg + Claetun + Glclar). The statistical design was completely randomized in factorial scheme 2 x 8 (two types of cuttings and eight types of inoculation, including control without AMF inoculation) with ten replications. The seedlings were kept in a greenhouse for 100 days and height was measured every 15 days to determine the absolute and relative growth rate (AGR and RGR). At the end of the experiment the seedlings were sacrificed and determined height, fresh and dry shoot mass and root and mycorrhizal colonization rate. The results indicate potential for production of Malpighia emarginata D.C. seedlings inoculated with AMF with tendency to reduce the time for transplanting. The Gimarg + Claetun combination promoted higher rates of absolute growth and height of seedlings from herbaceous cuttings. The species Glomus clarum, isolated or associated with C. etunicatum, promoted higher colonization rates in herbaceous and semi-hardwood seedlings, respectively.

Highlights

Soil microorganisms present potential for interaction with agricultural crops and promote their development (MACHINESKI et al, 2018)
The inoculation with Arbuscular mycorrhizal fungi (AMF) and its combinations influenced the height of the acerola cherry seedlings differently according to the type of cuttings (Figure 1a and b)
The treatment without AMF inoculation showed average height of seedlings higher than the other treatments, while the lowest heights were observed in the Glomus clarum treatments and in the combination G. margarita + G. clarum

Summary

Introduction

Soil microorganisms present potential for interaction with agricultural crops and promote their development (MACHINESKI et al, 2018). AMF are still mentioned for contributing to a decrease in the utilization of fertilizers, improved plant development and nutrition, shortening of the transplantation period, and increased survival of the seedlings in the field (VIERA et al, 2017). These nutritional effects and consequent plant development are due to the absorption of mineral nutrients, mainly phosphorus, nitrogen and water present in the soil that are captured by an extensive network of hyphae and transported to the plant. Several factors influence mycorrhizal symbiosis, including plant species, AMF species, climatic factors, soil fertility, pesticide management and use (ZOU et al, 2013)

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