Abstract
Mixed and single species pools of Culicoides were examined for virus isolation over a period of several years in Kenya. Two different strains of bluetongue virus and a strain of Nairobi sheep disease were obtained in BHK 21 C 13 cell cultures. Ephemeral fever virus was obtained from a mixed species pool, consisting almost entirely of C. shultzei, by the inoculation of 2-day-old infant mice. The trapping had been at a site where clinical ephemeral fever was occurring. A further 6 viruses were obtained by the inoculation of infant mice. Five of these appeared to be closely related by complement fixation and plaque inhibition tests and were members of the Palyam group. The remaining strain could not be identified as any known East African arbovirus and may be a new virus.
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