Arachidonic Acid Potentiates ACh Receptor Currents by Protein Kinase C Activation but Not by Receptor Phosphorylation

Abstract

The effects of arachidonic acid on ACh-gated channel currents were examined usingTorpedonicotinic ACh receptors expressed inXenopusoocytes. Arachidonic acid decreased ACh-evoked currents during treatment, to a greater extent in Ca2+-free extracellular solution. The currents were enhanced for more than 30 min after washing, reaching 150 and 170% in Ca2+-containing and -free extracellular solutions, respectively. The current enhancement was inhibited by the selective protein kinase C (PKC) inhibitor, GF109203X, whereas the current depression was not affected. Furthermore, arachidonic acid-evoked current depression was blocked in mutant ACh receptors with PKC phosphorylation site deletions on the α and δ subunits, but the long-lasting potentiation effect remained. These results indicate that arachidonic acid may decrease ACh receptor currents by a direct binding to PKC phosphorylation sites of the ACh receptors and may potentiate the currents via a novel pathway related to arachidonic acid-regulated PKC activation, but not via PKC phosphorylation of the ACh receptor itself.