Abstract
Studies were conducted to examine the metabolism of radioactively labelled arachidonic acid via the lipoxygenase and cyclooxygenase pathways and the metabolic conversions of radioactively labelled prostaglandin H 2; in the epidermal and dermal layers of the guinea-pig skin. Arachidonic acid was metabolized preferentially via lipoxygenase to hydroxyeicosatetraenoic acid (HETE). The major product of the cyclooxygenase pathway was prostaglandin D 2; prostaglandin E 2, was formed in lesser amounts. Epidermis exhibited much higher activities of these enzymes on a milligram protein basis than the dermis. In contrast, both skin layers showed the same very high activity of GSH-dependent prostaglandin H 2/prostaglandin D 2, isomerase; Prostaglandin D 2was virtually the only product formed by skin homogenates from prostaglandin H 2. Guinea-pig skin is a highly active site of arachidonic acid metabolism. These findings will provide the basis for pathobiochemical studies in inflammatory and hyperproliferative dermatoses.
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