Arachidonate 5-lipoxygenase-activating protein (ALOX5AP) gene rs4073259 polymorphism not associated with ischemic stroke in the Northeastern Chinese Han population☆

Abstract

ObjectiveAlthough recent evidence has implicated that 5-lipoxygenase activating protein (ALOX5AP) gene is associated with ischemic stroke (IS) risk, the underlying molecular mechanism remains to be defined. This study aimed to investigate the role of ALOX5AP rs4073259 in ischemic stroke in a Northeastern Chinese Han population. MethodsA total of 501 IS patients and 497 healthy controls were enrolled for polymerase chain reaction (PCR) and ligase detection reaction (LDR) analysis of ALOX5AP rs4073259 single nucleotide polymorphism (SNP). ResultsThere were no statistically significant differences in ALOX5AP rs4073259 allele and genotype frequencies between IS or subtypes of IS and controls. There was no significant difference in genotype and allele frequencies of atherosclerosis degree between ischemic subjects with carotid artery plaque or absence. However, total cholesterol (TC), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) in women patients were significantly higher than those in men (p=0.015, 0.000, and 0.008, respectively). Total homocysteine (tHcy) was higher in men patients than that in women (p=0.021). ConclusionThere was no statistically significant association of ALOX5AP rs4073259 SNP with ischemic stroke in this northeastern Chinese Han population living in Heilongjiang province, China.