Abstract
Arabinogalactan proteins (AGPs) are a highly diverse class of cell surface proteoglycans that are commonly found in most plant species. AGPs play important roles in many cellular processes during plant development, such as reproduction, cell proliferation, pattern formation and growth, and in plant-microbe interaction. However, little is known about the molecular mechanisms of their function. Numerous studies using monoclonal antibodies that recognize different AGP glycan epitopes have shown the appearance of a slightly altered AGP glycan in a specific stage of development in plant cells. Therefore, it is anticipated that the biosynthesis and degradation of AGP glycan is tightly regulated during development. Until recently, however, little was known about the enzymes involved in the metabolism of AGP glycans. In this review, we summarize recent discoveries of carbohydrate active enzymes (CAZy; http://www.cazy.org/) involved in the biosynthesis and degradation of AGP glycans, and we discuss the biological role of these enzymes in plant development.
Highlights
Arabinogalactan proteins (AGPs) are a family of proteoglycans found on the plasma membrane and in the cell walls of diverse species of plants
We summarize recent discoveries of carbohydrate active enzymes (CAZy; http://www.cazy.org/) involved in the biosynthesis and degradation of AGP glycans, and we discuss the biological role of these enzymes in plant development
Microbial Glycoside hydrolases (GHs) working on the degradation of plant AGPs have been reported in several studies, but little was known about the enzymes working on the biosynthesis and degradation of AGPs in plants
Summary
Arabinogalactan proteins (AGPs) are a family of proteoglycans found on the plasma membrane and in the cell walls of diverse species of plants. Tan et al (2010) proposed that the backbone is composed of a repeat of a β-1,3-galactotriose unit with or without side chains, which is connected by β-1,6-linkages (kinks) This model is based on the AGPs synthesized onto synthetic peptides expressed in tobacco cells and analyzed by NMR (Tan et al, 2004, 2010). Knowledge about each enzyme working on an individual step in the biosynthesis and degradation of AGP glycans is useful to understand the role of a particular sugar moiety of AGPs. This review outlines the recent findings for the carbohydrate active enzymes (CAZy; http://www.cazy.org/, Lombard et al, 2014) identified to be responsible for the biosynthesis and degradation of AGPs. The reader is referred to other excellent reviews for other topics with respect to structure, cell biological functions (Seifert and Roberts, 2007), localization, and commercial interests of AGPs (Nothnagel, 1997; Schultz et al, 1998; Majewska-sawka and Nothnagel, 2000; Gaspar et al, 2001; Showalter, 2001; Ellis et al, 2010; Tan et al, 2012). GLYCOSYLTRANSFERASES INVOLVED IN AGP BIOSYNTHESIS A large number of functionally distinct GTs are required for the biosynthesis of complex AGP glycans, e.g., www.frontiersin.org
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have