Arabidopsis glutamate receptor GLR3.7 is involved in abscisic acid response

Abstract

ABSTRACT The ionotropic glutamate receptor (iGluR) plays an important role in neuronal signaling in animal cells. There are at least 20 glutamate receptor-like (GLR) genes in Arabidopsis thaliana. These genes are involved in seed germination, root growth, wounding response, stomata closure, etc. A recent study showed that Arabidopsis clade III glutamate receptor GLR3.7 is involved in salt stress response. We tested whether GLR3.7 is involved in abscisic acid (ABA) response. In the present study, we found that the expression of GLR3.7 was reduced by ABA treatment. Under ABA-treated condition, GLR3.7 overexpression lines exhibited significantly higher seed germination rate at 60, 72 and 84 h under ABA-treated condition. A point mutation in 14-3-3 binding site of GLR3.7 in GLR3.7-S860A overexpression lines exhibited higher seed germination inhibition under ABA-treated conditions. Our results support that GLR3.7 is involved in ABA response in Arabidopsis. In addition, Ser-860 of GLR3.7 appears to be important in ABA response.