Arabidopsis G-Protein β Subunit AGB1 Negatively Regulates DNA Binding of MYB62, a Suppressor in the Gibberellin Pathway.

Xin Qi,Wensi Tang,Weiwei Li,Weiya Xu,Zhaoshi Xu,Zhijin Fan,Jun Chen,Ming Chen,Shiqin Gao,Youzhi Ma,Yongbin Zhou,Zhang He,Chunxiao Wang

doi:10.3390/ijms22158270

Abstract

Plant G proteins are versatile components of transmembrane signaling transduction pathways. The deficient mutant of heterotrimeric G protein leads to defects in plant growth and development, suggesting that it regulates the GA pathway in Arabidopsis. However, the molecular mechanism of G protein regulation of the GA pathway is not understood in plants. In this study, two G protein β subunit (AGB1) mutants, agb1-2 and N692967, were dwarfed after exogenous application of GA3. AGB1 interacts with the DNA-binding domain MYB62, a GA pathway suppressor. Transgenic plants were obtained through overexpression of MYB62 in two backgrounds including the wild-type (MYB62/WT Col-0) and agb1 mutants (MYB62/agb1) in Arabidopsis. Genetic analysis showed that under GA3 treatment, the height of the transgenic plants MYB62/WT and MYB62/agb1 was lower than that of WT. The height of MYB62/agb1 plants was closer to MYB62/WT plants and higher than that of mutants agb1-2 and N692967, suggesting that MYB62 is downstream of AGB1 in the GA pathway. qRT-PCR and competitive DNA binding assays indicated that MYB62 can bind MYB elements in the promoter of GA2ox7, a GA degradation gene, to activate GA2ox7 transcription. AGB1 affected binding of MYB62 on the promoter of GA2ox7, thereby negatively regulating th eactivity of MYB62.

Highlights

The heterotrimeric G protein pathway is a conservative transmembrane signal transduction pathway found in both animals and plants [1,2]
We found that following an increase in GA3 concentration, the plant height of AGB1 mutant partially recovered compared with the WT, indicating that AGB1 may be involved in the GA synthesis or degradation pathway
The results showed that without GA3 treatment, there were no significant differences in GA content between the mutants agb1-2 and N692967, and the WT (Figure 1C), but under GA3 treatment, the GA contents of agb1-2

Summary

Introduction

The heterotrimeric G protein pathway is a conservative transmembrane signal transduction pathway found in both animals and plants [1,2]. When the G protein pathway is activated, the GTP-G-α monomer is dissociated from the G-β-γ dimer, while the GTP-G-α monomer and the G-β-γ dimer interact with a variety of downstream effectors to transmit signals for different cell and physiological functions [4] (Anantharaman et al, 2011). Plants with mutated G protein complex components have altered morphology in their fruits, grain weight, roots, and leaves, and these mutants are sensitive to a variety of hormones, including IAA (auxin), GA (gibberellins), and BR (brassinosteroids) [2,5,10,11,12] The heterotrimeric G proteins are involved in growth and developmental processes such as seed germination and seedling development [5] (Ullah et al, 2003), cell division and morphology [6] (Ullah et al, 2002), ion channel regulation [7] (Assmann and Yu, 2015), stomatal development [8] (Wang et al, 2011), and the response to environmental conditions such as phytohormones, sugar, ROS (reactive oxygen species), and light [9] (Li et al, 2012).

Methods

Results

Conclusion