Arabidopsis CLP1-SIMILAR PROTEIN3, an Ortholog of Human Polyadenylation Factor CLP1, Functions in Gametophyte, Embryo, and Postembryonic Development

Denghui Xing,Qingshun Quinn Li,Hongwei Zhao

doi:10.1104/pp.108.129817

Denghui Xing, Qingshun Quinn Li + Show 1 more

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https://doi.org/10.1104/pp.108.129817

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Journal: Plant Physiology	Publication Date: Oct 29, 2008
Citations: 34	License type: CC BY 4.0

Affiliation: Miami University

Abstract

Polyadenylation factor CLP1 is essential for mRNA 3'-end processing in yeast and mammals. The Arabidopsis (Arabidopsis thaliana) CLP1-SIMILAR PROTEIN3 (CLPS3) is an ortholog of human hCLP1. CLPS3 was previously found to be a subunit in the affinity-purified PCFS4-TAP (tandem affinity purification) complex involved in the alternative polyadenylation of FCA and flowering time control in Arabidopsis. In this article, we further explored the components in the affinity-purified CLPS3-TAP complex, from which Arabidopsis cleavage and polyadenylation specificity factor (CPSF) subunits AtCPSF100 and AtCPSF160 were found. This result implies that CLPS3 may bridge CPSF to the PCFS4 complex. Characterization of the CLPS3 mutant revealed that CLPS3 was essential for embryo development and important for female gametophyte transmission. Overexpression of CLPS3-TAP fusion caused a range of postembryonic development abnormalities, including early flowering time, altered phyllotaxy, and abnormal numbers and shapes of flower organs. These phenotypes are associated with the altered gene expression levels of FCA, WUS, and CUC1. The decreased ratio of FCA-beta to FCA-gamma in the overexpression plants suggests that CLPS3 favored the usage of FCA regular poly(A) site over the alternative site. These observations indicate that Arabidopsis CLPS3 might be involved in the processing of pre-mRNAs encoded by a distinct subset of genes that are important in plant development.

Highlights

Prior to the genome era, prevalent assumption was that the more complicated an organism was, the more protein-coding genes the organism would possess
One states that the number of protein-coding genes is not significantly increased, the transcriptions of those protein-coding genes in higher eukaryotes are much more intensively regulated in term of both transcription level and the forms of their transcripts.[1,4]
One is that protein-coding genes are much intensively regulated at transcription level in higher eukaryotes

Summary

Introduction

Prior to the genome era, prevalent assumption was that the more complicated an organism was, the more protein-coding genes the organism would possess. Arabidopsis CLP1-SIMILAR PROTEIN3, an ortholog of human polyadenylation factor CLP1, functions in gametophyte, embryo and postembryonic development.

Results

Conclusion