Abstract
Aqueous two-phase extraction (ATPE) was used to extract bioactive compounds from haskap leaves (Lonicera caerulea) using salt/ethanol and sugar/propanol-based systems. The systems consisted of components that are generally recognized as safe (GRAS): ammonium sulphate/ethanol, sodium dihydrogen phosphate/ethanol, glucose/1-propanol, and maltose/1-propanol. The factors investigated were extraction time (5, 62.5, 120 min), sample loading (0.1, 0.55, 1 wt%), and tie line length (TLL) (low, medium, high) to represent different aqueous two-phase system (ATPS) compositions of salt or sugar and alcohol. Multi-response optimization was conducted to maximize the yield of chlorogenic acid, flavonoids, and total phenolic content. The results show that salt/ethanol ATPE systems had higher extraction efficiency than sugar/propanol ATPE. Among these four systems, sodium dihydrogen phosphate (NaH2PO4)/ethanol ATPE had the maximum extraction efficiency of 93.9% for chlorogenic acid, 96.8% for flavonoids, and 97.8% for total phenolic content. The corresponding partition coefficients were 1.73 for chlorogenic acid, 3.50 for flavonoids, and 6.59 for total phenolic content. Further analysis using high-performance liquid chromatography (HPLC) revealed that the extracts from ATPE were more refined in comparison to conventional solvent extracts. Additionally, nuclear magnetic resonance (NMR) spectra showed preliminary qualitative profiling of the extracts. This work demonstrates that ATPE using GRAS components is a green alternative to conventional extraction with organic solvents for the recovery of high-value bioactive compounds from haskap leaves.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.