Abstract
When trialkylamines are added to buffered solutions of peptides, unexpected adducts can be formed. These adducts correspond to Schiff base products. The source of the reaction is the unexpected presence of aldehydes in amines. The aldehydes can be detected in a few ways. Most importantly, they can lead to unanticipated results in proteomics experiments. Their undesirable effects can be minimized through the addition of other amines.
Highlights
Peptide mass shifts were found to be due to Schiff base reactions and were confirmed by accurate mass measurements
The existence of aldehydes in trialkylamine was verified with headspace GC-MS and Schiff’s test
Various conditions were tested for Schiff base reaction and tripropylamine-peptide reaction
Summary
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