Aqueous Extract Of Camellia Sinuses Shows Immunological And Histological Changes In Induced Inflammatory Animal Models

Abstract

The present study investigated the effect of green tea (Camellia sinuses) aqueous extract on the inflammatory response induced by Carrageenan (CGN) (1%) in Sprague dawaly rats. 48 rats were equally divided into 6 groups:control, green tea drinking, Carrageenan (1.2%) treated for 24 hours, green tea - Carrageenan treated for 24 hours, CGN treated for 72 hours, green tea - CGN treated for 72 hours. On the last day of drinking green tea aqueous extract, inflammation was induced to rats by Carrageenan . Twenty-four and seventy-two hours after CGN challenge, blood samples were withdrawn and animals were sacrificed. Animals which were injected with CGN had shown highly significant leucocytosis, monocytosis and eosinophilia. More reticuloendothelial organ damages like severe inflammation, cellular lymphocytic infilteration and congestion were distinguished in 72 hours animal group. Green teadrinking and CGN treated groups showed a significant improvement in reticuloendothelial organs such as thymus gland, spleen and liver. A histopathological improvement of these organs was observed in green tea and CGN 72 hours treated group more than that group which treated for 24 hours. This group showed also a significant drop in total leucocyte count and peritoneal fluid neutrophils while a significant increase of bone marrow lymphocyte count was observed when compared with the CGN treated animal group. A significant modulation in differential leucocytic count especially the drop in lymphocytic and eosinophilic percentage occurred. This was associated with lower serum globulin and immunoglobulin G (IgG) in green teadrinking-CGN treated animal group in comparison to CGN treated animal groups. This study explains the immunomodulatory role played by green tea in response to inflammatory immunostimulant agent.