Abstract

Reducing the pollution is a serious environmental challenge facing the oil producing industry worldwide. Conventionally, oil from oleaginous is extracted in large scale by mechanical expression or organic solvent extraction. In spite of its high yield, organic solvent extraction is time-consuming and very harmful to health and environment. Aqueous Enzymatic Extraction (AEE) is an eco-friendly extraction method using water as an extraction medium. Its application is a feasible alternative to traditional processing technologies without using organic solvents. However, its efficiency depends on many factors. This study aims to investigate the potential of AEE method to extract oil from safflower (Carthamus tinctorius L.) seeds using hemicellulase. Four extraction parameters with different levels that are incubation temperature, incubation time, initial pH, and enzyme concentration were evaluated. The optimum extraction conditions recorded were 65°C, pH 5, 4% w/w of enzyme and 3 hours of incubation. The obtained results showed that AEE reach a maximum of 70.45 % oil recovery. Therefore, Aqueous Enzymatic Extraction can be suggested as an environmentally cleaner alternative method to efficiently extract oil from safflower seeds.

Highlights

  • Safflower (Carthamus tinctorius L.), called false saffron, is an herbaceous plant belonging to the Asteraceae family

  • This study aims to investigate the potential of Aqueous Enzymatic Extraction (AEE) method to extract oil from safflower (Carthamus tinctorius L.) seeds using hemicellulase

  • The present study focused on evaluating the potential of AEE method, using hemicellulase enzyme, and determining extraction conditions that favor safflower oil recovery, which is a key step to optimize the overall process

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Summary

Introduction

Safflower (Carthamus tinctorius L.), called false saffron, is an herbaceous plant belonging to the Asteraceae family It has been cultivated since antiquity for its ornamental and dyeing properties in several regions of the world notably in Indian and Egyptian civilizations [1]. Safflower seed oil has a yellowish color and a slightly nutty flavor [4] Safflower oil is very rich in unsaturated fatty acids especially linoleic acid (approximately 80%) [5], which make it excellent for human consumption. Safflower oil fluidity and lightness properties make it ideal for cosmetic uses such as skin hydration and hair care It appears in the formulation of several dermatological and care body products. There is other industrial and technical applications of safflower oil such as varnish and biodiesel production [1,4,6]

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