Aquatic-Terrestrial Insecticide Fluxes: Midges as Neonicotinoid Vectors.

Abstract

Exposure of freshwater ecosystems to insecticides can negatively impact the development of emerging aquatic insects. These insects serve as an important nutritional subsidy for terrestrial insectivores. Changes in insect emergence phenology (i.e., emergence success and temporal pattern) or fluxes of insecticides retained by the emerging adults have the potential to negatively impact terrestrial food webs. These processes are influenced by contaminant toxicity, lipohilicity, or metabolic processes. The interplay between emergence phenology, contaminant retention through metamorphosis, and associated contaminant flux is not yet understood for current-use insecticides. In a microcosm study, we evaluated the impacts of a 24-h pulse exposure of one of three current-use insecticides, namely pirimicarb, indoxacarb, and thiacloprid, at two environmentally realistic concentration levels on the larval development and emergence of the nonbiting midge Chironomus riparius. In addition, we measured insecticide concentrations in the larvae and adults using ultrahigh performance liquid chromatography coupled to tandem mass spectrometry by electrospray ionization. Exposure to pirimicarb delayed larval development and emergence, and exposure to indoxacarb reduced emergence success. The neonicotinoid thiacloprid had the greatest impact by reducing larval survival and emergence success. At the same time, thiacloprid was the only insecticide measured in the adults with average concentrations of 10.3 and 37.3 ng/g after exposure at 0.1 and 4 µg/L, respectively. In addition, an approximate 30% higher survival to emergence after exposure to 0.1 µg/L relative to a 4-µg/L exposure resulted in a relatively higher flux of thiacloprid, from the aquatic to the terrestrial environment, at the lower exposure. Our experimental results help to explain the impacts of current-use insecticides on aquatic-terrestrial subsidy coupling and indicate the potential for widespread dietary exposure of terrestrial insectivores preying on emerging aquatic insects to the neonicotinoid thiacloprid. Environ Toxicol Chem 2023;42:60-70. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.