Abstract
ObjectiveOur research was to detect the expression of aquaporins. NF-κB in Irritable bowel syndrome (IBS) rat models’ colon so as to find novel pathogenesisof IBS.ResultsThe expression of AQP1, AQP3, and AQP8 of IBS model group was down-regulated while NF-κB p65 was up-regulated comparing with control group (p < 0.05), and the expression of AQP1, AQP3, and AQP8 of inhibitor group was up-regulated while NF-κB p65 was down-regulated comparing with IBS model group (p < 0.05).Materials and Methods18 adult female SD big rats were divided into three groups:the rats in control group were normal rats, the rats in IBS model group and the rats of inhibitor group were injected with the inhibitor of NF-κB (PDTC). Immunohistochemical technique and western blot were performed to detect the expression of AQP1, AQP3, AQP8 and NF-κB p65. RT-PCR was performed to detect the expression of AQP1, AQP3, and AQP8.ConclusionsLiquid water metabolic abnormalities and intestine permeability alteration might be the mechanism of IBS by down-regulating AQP1, AQP3 and AQP8 via NF-κB pathway.
Highlights
Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder, appearing as complex symptoms such as abdominal pain/discomfort [1]
The expression of AQP1, AQP3, and AQP8 of IBS model group was downregulated while NF-κB p65 was up-regulated comparing with control group (p < 0.05), and the expression of AQP1, AQP3, and AQP8 of inhibitor group was up-regulated while NF-κB p65 was down-regulated comparing with IBS model group (p < 0.05)
Liquid water metabolic abnormalities and intestine permeability alteration might be the mechanism of IBS by down-regulating AQP1, AQP3 and AQP8 via NF-κB pathway
Summary
Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder, appearing as complex symptoms such as abdominal pain/discomfort [1]. As a chronic biopsychological disorder, IBS is characterized by altered bowel habits excluding organic disorders. Except for the astrointestinal motility abnormality and distorted visceral perception of sensation, IBS is connected with several gastrointestinal and extraintestinal manifestations [2]. IBS is one of the most common disease, the etiology of the disease remains unknown. Several physical and psychological factors, such as stress, anxiety, and abnormal attitudes towards illness, are known to contribute to IBS’s pathogenesis [3]. Current evidence figures out that the diagnosis of IBS is still according to Rome IV and the actual quantity of the presence of such underlying cause of IBS , IBS’s pathogenesis remains unknown
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