Abstract
Background: Irritable bowel syndrome (IBS) is one of the most common gastrointestinal (GI) disorders. The cAMP-PKA pathway plays a key role in mediating cell responses to various stimuli both of which might play an important role in IBS. Objective: Our research was to detect the mechanism of IBS rat models via cAMP-PKA pathway so as to further detect the mechanism of IBS. Methods: IBS rats were built by stress and the conditioned stimulus. The study was divided into 3 groups( control group, model group, forskolin group ). The expressions of AQP1, AQP3, AQP8 and CREB(Serl33) in the colon of rats were detected by immunohistochemical technique. The expressions of AQP1, AQP3, AQP8, IL-1β,TGF β and TNF α in the colon were detected by PCR technique. Results: The IBS rats were built successfully. The expressions of AQP1, AQP3, AQP8 and CREB(Serl33) were down-regulated in the colon of IBS rats and were up-regulated with the intervention of the activator of AMP(p<0.05). The expressions of IL-1β TGF β and TNF α were up-regulated in the colon of IBS rats and were down-regulated with the intervention of the activator of AMP(p<0.05). Conclusions: The release of inflammatory cytokines, the activation of the immune system and liquid water metabolic abnormalities are the mechanisms of IBS via cAMP-PKA pathway. Funding Statement: This research was supported by Zhejiang Provincial Natural Science Foundation of China under Grant No.Y18H030008; and the Fund of Medicine and health science and technology plan projects in Zhejiang province (2017179719); Traditional Chinese medicine science and technology plan of Zhejiang province (2017ZA089); Traditional Chinese medicine science and technology plan projects of Zhejiang province (2016ZB071); and National Natural Science Foundation of China (81573760); and Traditional Chinese medicine science and technology plan projects of Zhejiang province (2015ZZ012); and medical health platform plan projects of Zhejiang province (2015RCA020); Zhejiang Provincial Natural Science Foundation of China (LY16H030010). Conflict of Interest: We have no conflict of interest. Ethics Approval Statement: This study was reviewed and approved by the Medical ethics committee of Zhejiang University.
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