Aptamer Functionalized ZnO Thin-Film Transistor Based Multiplexed Detection of Pb and E. Coli in Water

Abstract

In this work, we demonstrate the multiplexed detection platform to sense lead (Pb2+) and Escherichia coli in water using zinc oxide (ZnO) thin-film transistors (TFTs). Low-voltage ZnO TFTs fabricated by physical vapor deposition were functionalized with (3-Aminopropyl)triethoxysilane (APTES)–glutaraldehyde (GLU) molecules. TFTs with such functionalized ZnO surfaces showed good sensitivity and high specificity to the targets with a detection limit of 27 nM and <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$10^{{5}}$ </tex-math></inline-formula> colony forming unit per mL (cfu/mL) for Pb2+ and E. coli, respectively, with the TFT flat-band voltage ( <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">${V} _{\text {fb}}$ </tex-math></inline-formula> ) and, hence, the TFT transconductance, being dependent on the target concentration. The direct integration of the sensing with the TFT and associated integrated circuits promises high-density sensor arrays.