Abstract
We design a novel high throughput photonic crystal microsphere (PHCM) suspension array for multiplex mycotoxins in cereal samples based on the aptamer fluorescence signal recovery. The hybridization duplex strand DNA from mycotoxin aptamer and anti-aptamer respectively labeled with fluorescence dye and quencher was immobilized on the carboxylated surfaces of PHCMs. When the corresponding mycotoxin targets bind to their aptamers, the fluorescence recovery signal intensity of PHCMs reported the concentration of mycotoxins. The different kinds of mycotoxins were distinguished by the structure colors of PHCMs. The fluorescence signal intensity on the PHCMs is nearly higher 100 times than that of solid glass beads. The detection system presents an ultrasensitive, high selectivity, and small volume reagent screening for multiplex mycotoxins with a dynamic linear detection range of 0.1pg/mL–0.1ng/mL for AFB1/OTA and 0.1ng/mL–10ng/mL for FB1 and a limit of detection (LOD) of 15.96fg/mL, 3.96fg/mL and 11.04pg/mL for AFB1, OTA and FB1, respectively. The recovery rates in spiked cereal samples were well consistent with that of the traditional ELISA. The designed system provides a new high throughput aptamer-based suspension array for small molecule screening in parallel.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
